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名称：Zero Blunt® TOPO® PCR Cloning Kit without Competent Cells
货号：450245
规格：20T
价格：6330
保存：-20度
Zero Blunt® TOPO® PCR Cloning Kit without Competent Cells
Description
Zero Blunt® TOPO® PCR Cloning provides a highly efficient, 5 minute,
one-step cloning strategy ("TOPO® Cloning") for the direct insertion of
blunt-end PCR products amplified with proofreading thermostable polymerases into
a plasmid vector. Each kit has the Zero Blunt® TOPO® vector containing the ccdB
gene for positive selection, only permitting growth of plasmid vectors with
recombinants and are available with a variety of competent cells, or no competent cells,
depending on your needs and budget.
Fast and Easy—Go from PCR-to-clones in just 3 steps and in as little as 5 minutes hands-on time
Efficient—Achieve up to 95% clones with correct insert
Proven—Reliable performance for over a decade with over 4000 citations
Simple —No ligase, post-PCR procedures, or PCR primers containing specific sequences are required.
Zero Blunt® TOPO® Cloning Kits Overview
Specifications
General Specifications
PCR Enzyme: Proof-Reading Polymerase
Vector Type: TOPO Cloning Vectors,
Blunt DNA Cloning Vectors,
pCR
Product Size: 20 reactions
Cloning Method: Blunt TOPO®
Directionality: No
Replication Origin: pUC Origin
Bacterial or Yeast Strain: Not Included
Antibiotic Resistance (Bacterial): Zeocin™ (ZeoR),
Kanamycin (KanR)
Contents and Storage
Each Zero Blunt® TOPO® PCR Cloning Kit contains linearized and topoisomerase
I-activated pCR® Blunt II TOPO® Vector, dNTPs, Salt Solution, Control Template, and M13 Forward and Reverse Primers.
Store all components at -20 deg;C. All reagents are guaranteed stable for 6 months when properly stored. |