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产品名称：TOPO® TA Cloning® Kit for Sequencing without Competent Cells
产品货号：450030
产品规格：20T
产品价格：6110
TOPO® TA Cloning® Kit for Sequencing without Competent Cells
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning")
for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the
pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector
sequence from each reaction and are available with a variety of competent cells, or no competent cells, depending on your needs and budget.
Get More Sequence—Allows for more insert sequence and less vector sequence when using standard sequencing primers
Fast and Easy—Go from PCR-to-clones in just 3 steps and in as little as 5 minutes hands-on time
Efficient—Achieve up to 95% clones with correct insert
Proven—Reliable performance for over a decade with over 4000 citations
TOPO® TA Cloning® Kits for Sequencing Kit Overview
VECTOR: pCR™4-TOPO® TA Vector
Optimized cloning vector for improved sequencing results View More
CLONING METHOD: TOPO® TA Cloning
Topoisomerase I based 5 minute ligation of PCR products with 3´A overhangs (Taq polymerase amplified) to TOPO® vector with T overhangs
COMPETENT CELLS: Various Options
Choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing competent cells, or use your own
pCR™4-TOPO® TA Vector—Optimized for Sequencing
We have removed much of the multiple cloning site from the pCR™4-TOPO® TA vector to shorten the distance between sequencing primer
sites and the insert site to as little as 33 bp. This means sequencing reactions give less vector sequence and more insert sequence.
The pCR™4-TOPO® TA vector has sites for 4 common sequencing primers: M13 forward, M13 reverse, T7, and T3. The kits |