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alpha-Glucosidase Assay

产品编号: 11742027001     查看说明书
产品名称: alpha-Glucosidase Assay  .0   订购此产品 
供应商: Roche
规格: 1 kit for 30 tests and 10 blanks
目录价: 4957
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应用范围: 葡萄糖酶检测
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Application
Fast, sensitive and specific colorimetric assay for the quantitative determination of neutral α-glucosidase in life science research applications. The measurement is performed exclusively in human semen samples as a parameter for the evaluation of male infertility.
Contents
Reaction Buffer
Substrate Solution containing 4-nitrophenyl-α-D-glucopyranoside
Stopping Buffer
Castanospermine
Standard Solution containing 4-nitrophenol
Principle
The sample is incubated at neutral pH in the presence of SDS assuring that only neutral α-glucosidase from epididymis is measured. α-Glucosidase cleaves 4-nitrophenol from the substrate 4-nitrophenyl-α-D-glucopyranoside yielding a yellow color which can be measued in cuvettes or microplates.
Note: Contains castanospermine (α-glucosidase inhibitor) for the preparation of the blank.
Quality
The assay is linear between 2 mU/ml and 45 mU/ml. Intra-assay variances are only 1 - 3%; inter-assay variances are up to 5%.
α-Glucosidase Assay
11742027001 1 kit for 30 tests and 10 blanks
名称 产地 货号 规格 价格
α-Glucosidase Assay ROCHE 11742027001 30t 4724

保存条件: 常温
说明书地址: 点击查看详细
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