名称：pENTR™/TEV/D-TOPO® Cloning Kit with One Shot® TOP10 Chemically Competent E. coli货号：K252520规格：20 reactions价格：4540The pENTR™⁄TEV⁄D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™⁄TEV⁄D-TOPO® entry vector at greater than 90% efficiency, and include a TEV protease-dependent cleavage site for removal of N-terminal tags from expressed proteins. The kit comes with everything necessary to clone and select your PCR amplified gene of interest。Optimized pENTR™ ⁄TEV⁄D-TOPO® Vector。The pENTR™ ⁄TEV⁄D-TOPO® vector includes a Tobacco Etch Virus (TEV) recognition site for protease-dependent cleavage of an N-terminal tag from your recombinant protein. The vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site. This allows clones to be easily sequence verified and recombined into your choice of attR containing Gateway® destination vectors. A Kanamycin resistance gene and a pUC origin are used for selection and high-copy propagation in E. coli.