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SuperScript® III CellsDirect™ cDNA Synthesis Kit
The SuperScript® III CellsDirect cDNA Synthesis Kit is optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA. Lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning and PCR. For real-time quantitative RT-PCR, see the note below.
Advantages of the SuperScript® III CellsDirect cDNA Synthesis Kit include:
• Compatible with a wide range of mammalian cell types grown under different treatment conditions
• Single-tube format minimizes reagent loss, sample loss, and handling time
• Total lysate volume is used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts
• SuperScript® III Reverse Transcriptase, with reduced RNase H activity and higher thermal stability, produces high yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection
Reverse Transcriptase: SuperScript® III
Downstream Application: RT-PCR (Endpoint),
Real-Time PCR
Reaction Format: Separate Components
Sample Type (General): Cells
Product Size: 25 reactions
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature: 50° C
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