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产品名称:MRVP Medium
供应商:Oxoid
货号:CM0043
规格:500g
产品说明：
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-6°C.
Technique
Inoculate a 10ml tube of MRVP Medium with two loopfuls of a pure, 4-6 hours old, Peptone Water CM0009 culture of the organism under test.
Incubate not less than 48 hours at 35°C for the MR test but more usually 3-5 days at 30°C. A heavy inoculum and 18-24 hours incubation at 35°C may give a rapid result 10. A rapid VP test may be carried out from a heavy inoculum and incubation in a water bath at 35°C for 4-5 hours. Some organisms (Hafnia alvei) require incubation at 25°C to give a positive VP test.
Directions
Add 17g to 1 litre of distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes. |