PageRulerPrestained Protein Ladder
SM0671
2 x 250 μl(for 100 mini gel applications 5 μl per well or 50 large gel applications 10 μl per well)
Lot: Expiry Date:
Store at -20°C(or at 4°C for 3 months)
Description
PageRuler? Prestained Protein Ladder is a 3-color
protein molecular weight standard containing 10
prestained recombinant proteins covering a wide range
molecular weights from 10 kDa to 170 kDa. The ladder
contains one orange reference band of ~70 kDa and one
green reference band of 10 kDa.
Lot-to-lot variation of the apparent molecular weight of
prestained proteins is ~5%.
The ladder is supplied in gel loading buffer and is
ready-to-use: no heating, further dilution or addition of a
reducing agent is required.
Applications
Monitoring of protein separation during SDS-PAGE (1).
Verifying Western transfer efficiency (2, 3).
Approximate sizing of proteins on SDS-polyacrylamide
gels and Western blots.
Locating a protein of interest for excision from an
unstained preparative gel.
Storage Buffer
62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA,
2% (w/v) SDS, 10 mM DTT, 1 mM NaN3 and
33% (v/v) glycerol).