中文名：抑肽素，胃蛋白酶抑制剂
英文名：Pepstatin，Pepstatin A
PN-Isovaleryl-L-valyl-L-valyl-3-hydroxy-6-methyl-gamma-aminoheptanoyl-L-alanyl-3-hydroxy-
6-methyl-gamma-aminoheptanoic acid，
(3S,4S)-3-Hydroxy-4-[[(2S)-2-[[(3S,4S)-3-hydroxy-6-methyl-4-[[(2S)-3-methyl-2-[[(2S)-3-methy
l-2-(3-methylbutanoylamino)butanoyl]amino]butanoyl]amino]heptanoyl]amino]propanoyl]amin
o]-6-methylheptanoic acid; Pepstatin A
分子式:C34H63N5O9
分子量:685.89
Sigma相关资料如下：
P4265 Sigma Pepstatin A
microbial, ≥75% (HPLC)
CAS Number: 26305-03-3
Linear Formula: C34H63N5O9
Molecular Weight: 685.89
Beilstein Registry Number: 2201362
EC Number: 247-600-0
MDL number: MFCD00060740
PubChem Substance ID: 24898519
Description
Preparation Note Pepstatin can be dissolved at 1 mg/ml in 10% (v/v) acetic acid in methanol (9:1 methanol:acetic acid). The inclusion of acetic acid may be necessary to dissolve this peptide in methanol or DMSO. It has been dissolved at 1 to 2 mg/ml in ethanol, but heat may be required for complete dissolution. Solutions of Pepstatin A can be heated as high as 60 °C without any decomposition of the peptide.
Stock solutions at 1 mg/ml are stable at least a week at 4 °C. A 1 mM solution in methanol or DMSO is stable for months at -20 °C. If solutions become darker yellow, the reagent is hydrolyzing.
A working concentration of 1 μM is stable for at least one day at room temperature.
Biochem/physiol Actions Pepstatin A is an inhibitor of acid proteases (aspartyl peptidases). It forms a 1:1 complex with proteases such as pepsin, renin, cathepsin D, bovine chymosin, and protease B (Aspergillus niger). The inhibitor is highly selective and does not inhibit thiol proteases, neutral proteases, or serine proteases. Solubilized γ-secretase and retroviral protease are also inhibited by Pepstatin A. It has been used to characterize proteases from several sources.
性质
biological source microbial
assay ≥75% (HPLC)
mp 233 °C (dec.)(lit.)
storage temp. 2-8°C